| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143759 |
| Chromosome: | chromosome 12 |
| Location: | 7357772 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557050 | CPLD8,YGG2 | Conserved expressed protein with YGGT domain; (1 of 2) K02221 - YggT family protein (yggT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTACCTGAACCTGTTCCGCGGCCTCATC |
| Internal bar code: | GGGTTGGAACTCGCGCCGAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 699 |
| LEAP-Seq percent confirming: | 99.759 |
| LEAP-Seq n confirming: | 414 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCAGAGGGCAAGGACAG |
| Suggested primer 2: | CCTGTGAGTTGTTGAGGGGT |