| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143770 |
| Chromosome: | chromosome 9 |
| Location: | 3193317 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387393 | (1 of 4) K06067 - histone deacetylase 1/2 (HDAC1_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTGTTTGGAGTGCCGGCAATCAACAAT |
| Internal bar code: | ACCTAGCGTGTCAGTCCCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 98.8662 |
| LEAP-Seq n confirming: | 436 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTAGTGCAAAGGTGCAG |
| Suggested primer 2: | GTTGTGACGCAGTATGCCAC |