| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143791 |
| Chromosome: | chromosome 16 |
| Location: | 590764 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692300 | (1 of 6) 3.6.3.43 - Peptide-transporting ATPase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACGAAGTTGCGGAGACGGCTCGGGTCT |
| Internal bar code: | GCTCTTCGGTGCTGCATGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 61.8072 |
| LEAP-Seq n confirming: | 513 |
| LEAP-Seq n nonconfirming: | 317 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGTCGCAGTGCTGAATG |
| Suggested primer 2: | TCGCGACGTCACAAAGATAG |