| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143806 |
| Chromosome: | chromosome 10 |
| Location: | 277611 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419700 | CGL6 | (1 of 4) PF05653 - Magnesium transporter NIPA (Mg_trans_NIPA); Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCAAACACCCCTTCCTTTGGCCCTTCT |
| Internal bar code: | CCCGCCCCGGGCAACTGGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 162 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGGGGAACTGAAAACAG |
| Suggested primer 2: | GGTTCTCCATACCGTGCTGT |