| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143813 |
| Chromosome: | chromosome 8 |
| Location: | 3537164 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375400 | GSM1 | (1 of 1) PTHR11850//PTHR11850:SF102 - HOMEOBOX PROTEIN TRANSCRIPTION FACTORS // SUBFAMILY NOT NAMED; Gamete-specific minus 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAATGCCTGCGCTGCCGCACCGCCCAAT |
| Internal bar code: | GCTTCTGTGCTCTTTCTCCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1059 |
| LEAP-Seq percent confirming: | 99.6504 |
| LEAP-Seq n confirming: | 5415 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGTTGCAATGGCCTAGT |
| Suggested primer 2: | CCGGTTTTATGCTGTCAGGT |