| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143816 |
| Chromosome: | chromosome 14 |
| Location: | 4027827 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633500 | (1 of 1) IPR024224 - DENND6 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTGCCCAGCTGGGCACCAGCCGAGTGA |
| Internal bar code: | CGTTCTGAACAACTTAGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 97.2099 |
| LEAP-Seq n confirming: | 5052 |
| LEAP-Seq n nonconfirming: | 145 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGAAGAGGTCGTCTAGG |
| Suggested primer 2: | AGCCTCTCTTTCGCATACCA |