| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.143817 |
| Chromosome: | chromosome 2 |
| Location: | 3197060 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095069 | (1 of 1) PTHR10681//PTHR10681:SF119 - THIOREDOXIN PEROXIDASE // PEROXIREDOXIN DOT5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTCGATTCCGGAGAGGGGGTGCATGCA |
| Internal bar code: | GTGGCCCAACGCGTAGCGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 347 |
| LEAP-Seq percent confirming: | 99.8578 |
| LEAP-Seq n confirming: | 14048 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACCGGCTATTCAACAAC |
| Suggested primer 2: | ACCTTCCAAACGAACCTCCT |