| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143836 |
| Chromosome: | chromosome 12 |
| Location: | 8849460 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544802 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGTTCAAGTTTGCAAACGTATGTGCCG |
| Internal bar code: | TACGCTTATTTTTCAGACATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 95.8854 |
| LEAP-Seq n confirming: | 1305 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACATGCGCCTTCTCTCA |
| Suggested primer 2: | TAGTCATCAAATGCGAAGCG |