| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143869 |
| Chromosome: | chromosome 12 |
| Location: | 2580144 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505250 | FAP288 | (1 of 3) PF13202//PF13499 - EF hand (EF-hand_5) // EF-hand domain pair (EF-hand_7); EF Hand Containing Flagellar Associated Protein 288 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACCAAGAGCCGGCTAGTTAAATGCATG |
| Internal bar code: | GGGGGAGAAGAAACCCCGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 568 |
| LEAP-Seq percent confirming: | 99.9515 |
| LEAP-Seq n confirming: | 2062 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGACATCCTTCTGGGGC |
| Suggested primer 2: | GTTTCAGGTGTGGCAATGTG |