Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.143909 |
Chromosome: | chromosome 2 |
Location: | 6021314 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g112400 | CHI1 | (1 of 1) K12310 - Di-N-acetylchitobiase (CTBS); Chitinase-like hydrolase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGCAAGGCCCTGTGTATTGTGTAACGG |
Internal bar code: | CTGGGCCAGCAAATACTGACTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 770 |
LEAP-Seq percent confirming: | 99.7133 |
LEAP-Seq n confirming: | 1739 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGCAACAGCACTATCGCAT |
Suggested primer 2: | GAGTGCAAACTGCTGGTGAA |