| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143925 |
| Chromosome: | chromosome 7 |
| Location: | 2333180 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328000 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCTCCTTACACAACGCCAAGCTTACAT |
| Internal bar code: | CGTGGCATGTGCCGACTTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 99.291 |
| LEAP-Seq n confirming: | 2941 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAAGTTGCAAACAGAAAA |
| Suggested primer 2: | ACTACCGAACAGGGTTCACG |