| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.143962 |
| Chromosome: | chromosome 9 |
| Location: | 3514446 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389801 | (1 of 1) PF08854 - Domain of unknown function (DUF1824) (DUF1824) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTACGTGGTGTATGTGTGTGTGTGTGTG |
| Internal bar code: | ATCGTTAGTCTGCTGCCCTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2 |
| LEAP-Seq percent confirming: | 73.0769 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTCGCAGGTACCCTTAG |
| Suggested primer 2: | ATACCTGCTTATCGCGCATT |