| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.143980 |
| Chromosome: | chromosome 12 |
| Location: | 3668688 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514400 | (1 of 1) PTHR12802//PTHR12802:SF60 - SWI/SNF COMPLEX-RELATED // PROTEIN REVEILLE 1-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGTACAATTGTGCGCAGCGGCCATCGA |
| Internal bar code: | AGAAATCTCGTAGCTTAGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 427 |
| LEAP-Seq percent confirming: | 99.809 |
| LEAP-Seq n confirming: | 1045 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTAGTCGGTATTGGAGA |
| Suggested primer 2: | AAGGCACAGTGGACGGTATC |