| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143991 |
| Chromosome: | chromosome 9 |
| Location: | 6746449 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409100 | HEMD,UROS1,UPS1,UROS | Uroporphyrinogen-III synthase; (1 of 1) 4.2.1.75 - Uroporphyrinogen-III synthase / Uroporphyrinogen-III cosynthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGCGCGTGGGTGTATTGCAATACCGTT |
| Internal bar code: | CAGCCGGGGAACGTTGCAGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 98.5611 |
| LEAP-Seq n confirming: | 3151 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTATCTCGCTTGCTGGCAT |
| Suggested primer 2: | AATCTCGATGGCGTAAGTGG |