| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.143994 |
| Chromosome: | chromosome 14 |
| Location: | 1055272 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGTACGCATGAAGATATGCCATGACCGC |
| Internal bar code: | GTGGTGTGCGAGGGTCAGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 91.0448 |
| LEAP-Seq n confirming: | 2684 |
| LEAP-Seq n nonconfirming: | 264 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCAAACTGCATATAAGA |
| Suggested primer 2: | TGAGATTGATGGCGAGAGTG |