| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144006 |
| Chromosome: | chromosome_7 |
| Location: | 5173981 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g348400 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGCTTGATCGCCAGCAGCAGCGCCGTGGA |
| Internal bar code: | ACCGAGCCTCGGGCTCATGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 89.6191 |
| LEAP-Seq n confirming: | 47102 |
| LEAP-Seq n nonconfirming: | 5456 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGTGGTTGTGGTTGTTG |
| Suggested primer 2: | TGCGTGTGAGAAGTGGAGTC |