| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144043 |
| Chromosome: | chromosome_8 |
| Location: | 3806773 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g377350 | FAP121,FAP180,ADK5 | Adenylate kinase; Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAAGGGGCGTGGGGAAGGGGTGAAGGGGCC |
| Internal bar code: | CATCATCCACTGCAGTACCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 99.4924 |
| LEAP-Seq n confirming: | 1568 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCTGAAAAGCAAGGAAG |
| Suggested primer 2: | TGAGTGCATCTGTGCTTTCC |