| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144046 |
| Chromosome: | chromosome_10 |
| Location: | 4049336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g449250 | KAP1,FLA3 | Kinesin-II-associated protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCACCTCACCTTGTACGCCGGAGCCTGCGC |
| Internal bar code: | GATTATGAGGTATGTGCACCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 99.529 |
| LEAP-Seq n confirming: | 1268 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCTCAAGTTCGAGTCC |
| Suggested primer 2: | ATGCGTAGATGACCCTGACC |