Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.144046 |
Chromosome: | chromosome_10 |
Location: | 4049337 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g449250 | KAP1,FLA3 | Kinesin-II-associated protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAGTTTTAGTTGTAGCTGTACGGATGGTGC |
Internal bar code: | GGGAGGGTAATGGAATGGCCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 771 |
LEAP-Seq percent confirming: | 96.9768 |
LEAP-Seq n confirming: | 19054 |
LEAP-Seq n nonconfirming: | 594 |
LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAACCTCAAGTTCGAGTCC |
Suggested primer 2: | ATGCGTAGATGACCCTGACC |