| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144054 |
| Chromosome: | chromosome 14 |
| Location: | 305657 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609900 | (1 of 2) PTHR23130//PTHR23130:SF61 - FAMILY NOT NAMED // DEFENSE PROTEIN L(2)34FC | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTACCGTCAGGACTGCTAAACAGGAAG |
| Internal bar code: | CAATGGTGGGACATGGCGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 99.9577 |
| LEAP-Seq n confirming: | 2363 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGTGCATGACTGGATGG |
| Suggested primer 2: | GTTGAATCACAGTGCGGTTG |