| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144065 |
| Chromosome: | chromosome 14 |
| Location: | 3236955 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629283 | (1 of 1) 3.5.4.34 - tRNA(Ala)(adenine(37)) deaminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTATTCAGAGGCGCCCCGGCCGGTCGT |
| Internal bar code: | ACCAGATTTTGAAACGTGCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 50 |
| LEAP-Seq percent confirming: | 9.52381 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTCAAGGGAATGAGGTC |
| Suggested primer 2: | CCTCAAAGACGGATACTGGC |