Insertion junction: LMJ.RY0402.144092_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.144092
Chromosome:chromosome 7
Location:4580282
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g344050 (1 of 1) K14555 - U3 small nucleolar RNA-associated protein 13 (UTP13, TBL3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGACGAAGATGAGGATGATGAGGAGGAT
Internal bar code:AGCCAGTGGAGGCCGAATATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:157
LEAP-Seq percent confirming:92.6554
LEAP-Seq n confirming:328
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGAGGAGGAGGAGGAGGA
Suggested primer 2:CACACAACCACACACCACAA