| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144096 |
| Chromosome: | chromosome 4 |
| Location: | 3917993 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g230830 | (1 of 1) IPR000104//IPR001012//IPR029071 - Antifreeze protein, type I // UBX domain // Ubiquitin-related domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCCAGGCGTGCGTACTGTGACCCTGGAC |
| Internal bar code: | GACGTTCGCTCGACGCGCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 833 |
| LEAP-Seq percent confirming: | 87.3724 |
| LEAP-Seq n confirming: | 39190 |
| LEAP-Seq n nonconfirming: | 5664 |
| LEAP-Seq n unique pos: | 144 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCATGCTGCTCTGTGACT |
| Suggested primer 2: | ATGAGGGACGCCTAGAGACA |