| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144097 |
| Chromosome: | chromosome 14 |
| Location: | 282728 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCAACCACATACGAGCATGAAGTGCAC |
| Internal bar code: | GTTGCGAGTCCCTACACGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 99.5413 |
| LEAP-Seq n confirming: | 651 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGCAAGGGAAATG |
| Suggested primer 2: | TGACGTCGGGGTAGTAGGTC |