| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144097 |
| Chromosome: | chromosome 14 |
| Location: | 282729 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATGCGTGGTTGGTGGATGAGGACGAGG |
| Internal bar code: | AGGTGCGAGGGAAAGCAAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 668 |
| LEAP-Seq percent confirming: | 99.7439 |
| LEAP-Seq n confirming: | 1558 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGCAAGGGAAATG |
| Suggested primer 2: | TGACGTCGGGGTAGTAGGTC |