| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144137 |
| Chromosome: | chromosome 2 |
| Location: | 8480657 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141786 | PDE25 | 3\',5\'-cyclic-nucleotide phosphodiesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGTGGGCTGNTGCCATCTGTCTGGAA |
| Internal bar code: | TCCTGGAAGCGCGCAATGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 66 |
| LEAP-Seq percent confirming: | 7.27273 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGCACTTCTCGCTTCTC |
| Suggested primer 2: | GCTATGTGGGAGTCGGTGTT |