| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144179 |
| Chromosome: | chromosome_11 |
| Location: | 393389 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g467580 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGAGAAGAAGTTTGGTGCATTTGCAGAAT |
| Internal bar code: | TTGGCACCCCGAGTAAAGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 394 |
| LEAP-Seq percent confirming: | 98.8229 |
| LEAP-Seq n confirming: | 1763 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTAACGAAACAAGCCCC |
| Suggested primer 2: | TGGTCTATTTTGGGATGGGA |