| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144206 |
| Chromosome: | chromosome 12 |
| Location: | 3439992 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496400 | (1 of 2) 2.7.7.52 - RNA uridylyltransferase / TUT | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCGCGGCGCGGGGGCGCTGGCCGGTGC |
| Internal bar code: | CCTGAACAGGGACAGCGCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 141 |
| LEAP-Seq percent confirming: | 96.8 |
| LEAP-Seq n confirming: | 484 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCATAATAGTCGATGCCA |
| Suggested primer 2: | TTGCCCTTGTTCGTAGGTTC |