Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.144227 |
Chromosome: | chromosome 13 |
Location: | 2457052 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g579901 | DEG6 | (1 of 2) PTHR22939:SF67 - PROTEASE DO-LIKE 9; Deg protease | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGGCAATGCCCACGCACTGCCCGTCGT |
Internal bar code: | GTTGGGTTCGGTTTTTCGAGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 388 |
LEAP-Seq percent confirming: | 99.2809 |
LEAP-Seq n confirming: | 2209 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGACCAGATCCAGGCACAT |
Suggested primer 2: | GGTTCTCAAGCTTCTGCCAC |