Insertion junction: LMJ.RY0402.144227_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.144227
Chromosome:chromosome 13
Location:2457052
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g579901 DEG6 (1 of 2) PTHR22939:SF67 - PROTEASE DO-LIKE 9; Deg protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAGGCAATGCCCACGCACTGCCCGTCGT
Internal bar code:GTTGGGTTCGGTTTTTCGAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:388
LEAP-Seq percent confirming:99.2809
LEAP-Seq n confirming:2209
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GAGACCAGATCCAGGCACAT
Suggested primer 2:GGTTCTCAAGCTTCTGCCAC