Insertion junction: LMJ.RY0402.144237_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.144237
Chromosome:chromosome 2
Location:3975016
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g096737 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGCATTGATGACCACTCTCTTCCCTAGG
Internal bar code:CGGGGAATTATGGTCGCTGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:746
LEAP-Seq percent confirming:97.8613
LEAP-Seq n confirming:6040
LEAP-Seq n nonconfirming:132
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCACTTTGTTTCCTACCC
Suggested primer 2:TCTGTACCGACAAGGTGCTG