Insertion junction: LMJ.RY0402.144240_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.144240
Chromosome:chromosome 9
Location:1434267
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g398450 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTAAACATGAGCTGCGTGCGCAAAGTGG
Internal bar code:GGGCGTACGCATTAGACCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:668
LEAP-Seq percent confirming:99.7782
LEAP-Seq n confirming:5398
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:AAGACAGGCCGCTGCTAATA
Suggested primer 2:CCCAAACTAAAGCAAACCCA