| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144267 |
| Chromosome: | chromosome 12 |
| Location: | 594376 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAATTGCTGGGCCGCTTATAGAAACCTC |
| Internal bar code: | TTATAGGGTGGGGCCTGCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 69.1589 |
| LEAP-Seq n confirming: | 296 |
| LEAP-Seq n nonconfirming: | 132 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGTGTGCTCCTTGTGTC |
| Suggested primer 2: | ACGGTCCATGATATCCCAAA |