| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144277 |
| Chromosome: | chromosome 6 |
| Location: | 4929045 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279474 | MUT68 | (1 of 12) 2.7.7.19 - Polynucleotide adenylyltransferase / RNA adenylating enzyme; Terminal uridyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTCCCCGGGGCGGAGGCAGTTGGGGGG |
| Internal bar code: | TGGCGACACCGCCCCCAGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 255 |
| LEAP-Seq percent confirming: | 99.7492 |
| LEAP-Seq n confirming: | 5171 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTTGCATTTTGCACAGA |
| Suggested primer 2: | TACAGTCAAGGCAAAACCCC |