| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144281 |
| Chromosome: | chromosome 12 |
| Location: | 2426041 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507051 | (1 of 2) K01193 - beta-fructofuranosidase (E3.2.1.26, sacA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGGGTCGGTGGTCGCCGCCCCGGGCTT |
| Internal bar code: | AAAATAGTTAGGGTACAGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 264 |
| LEAP-Seq percent confirming: | 88.1577 |
| LEAP-Seq n confirming: | 11092 |
| LEAP-Seq n nonconfirming: | 1490 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGACTGCTTGCATTACGGA |
| Suggested primer 2: | CATATGAGATTGTGCGTGCC |