Insertion junction: LMJ.RY0402.144285_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.144285
Chromosome:chromosome 17
Location:6208457
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g741950 BBS1 Bardet-Biedl syndrome-1 associated protein; (1 of 1) K16746 - Bardet-Biedl syndrome 1 protein (BBS1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACGCTTCCATACACAAGAACTCATACACG
Internal bar code:CCCAGTGCATATTGCCTCTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:953
LEAP-Seq percent confirming:99.7007
LEAP-Seq n confirming:5662
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CACCAGGTGATCGTTGTCAC
Suggested primer 2:GTCGTTTTGTCCCACTCGAT