| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144299 |
| Chromosome: | chromosome_3 |
| Location: | 4764723 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g178150 | RSP20,CAM1 | Calmodulin, radial spoke protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCCCGAAAAATGGCCGCGAACACCGAGCA |
| Internal bar code: | CTACATTTCGTCGTTGAGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1019 |
| LEAP-Seq percent confirming: | 99.7846 |
| LEAP-Seq n confirming: | 8802 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTAGGACACGGTCAAT |
| Suggested primer 2: | GTTGATAGCATGAGGCAGCA |