| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144312 |
| Chromosome: | chromosome 7 |
| Location: | 5659406 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352300 | (1 of 3) 2.1.1.200 - tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase / TrMet(Xm32) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCTCAATCCTGCCCTGCCGTTGCCAAT |
| Internal bar code: | GCCTGTGGGTGCGCGTCTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 925 |
| LEAP-Seq percent confirming: | 32.2888 |
| LEAP-Seq n confirming: | 711 |
| LEAP-Seq n nonconfirming: | 1491 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTACTCGGATAAATGCGG |
| Suggested primer 2: | GACACCGACCCCAACTTCTA |