| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144326 |
| Chromosome: | chromosome 17 |
| Location: | 4263377 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730600 | (1 of 6) 3.2.1.4 - Cellulase / Endoglucanase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCAGCAACCGCTCCATCTGCGCCACCC |
| Internal bar code: | TGTCTGTCCTAACCATCGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1126 |
| LEAP-Seq percent confirming: | 63.2805 |
| LEAP-Seq n confirming: | 10652 |
| LEAP-Seq n nonconfirming: | 6181 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATACCGTGGCCTATGCT |
| Suggested primer 2: | AGAAAGGAGGAGAGGGCTTG |