| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144346 |
| Chromosome: | chromosome 2 |
| Location: | 866022 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079150 | (1 of 1) PF03031//PF13671 - NLI interacting factor-like phosphatase (NIF) // AAA domain (AAA_33) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGCGGACTTGTGCTCAGACTTGCGTGC |
| Internal bar code: | ATGAAACGCCTCCAGTTCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 733 |
| LEAP-Seq percent confirming: | 99.702 |
| LEAP-Seq n confirming: | 7361 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCAGCTTTACGGAGGCT |
| Suggested primer 2: | CCTGGCTTCTGAGGTACAGC |