Insertion junction: LMJ.RY0402.144351_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.144351
Chromosome:chromosome 6
Location:5766097
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g287300 COG9 ankyrin repeat protein; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGACCTACCCCGCGTACGCGTCAATACA
Internal bar code:GGACGCGTCTGGCTCCAAGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:146
LEAP-Seq percent confirming:99.763
LEAP-Seq n confirming:842
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TAAAGGACGGGCTCATCATC
Suggested primer 2:CAGGCTCGCTTCCTATTGAC