| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144366 |
| Chromosome: | chromosome 8 |
| Location: | 572682 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359500 | (1 of 1) 6.3.2.4 - D-alanine--D-alanine ligase / D-alanylalanine synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATCTCAGCTCGTTCCGTGTTGTGGCCT |
| Internal bar code: | ACGTTAGGTTCTAAAAATTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 99.776 |
| LEAP-Seq n confirming: | 1782 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCCGTATACACGCACAC |
| Suggested primer 2: | GGCAGGAGATGATGTGTGTG |