| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144369 |
| Chromosome: | chromosome 11 |
| Location: | 463313 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467592 | Probable amino acid/metabolite permease; (1 of 1) PTHR11785//PTHR11785:SF228 - AMINO ACID TRANSPORTER // GABA-SPECIFIC PERMEASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCATTGGCGGGCGTGAGGTCACGCCGCA |
| Internal bar code: | GGGGCGGGGGTTGGCTTCCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 97.7778 |
| LEAP-Seq n confirming: | 132 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCTACGTGGGTTGCATA |
| Suggested primer 2: | GAGGACCAGGTGTGTGTGTG |