| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144375 |
| Chromosome: | chromosome 6 |
| Location: | 2959899 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273100 | (1 of 3) PF03067 - Chitin binding domain (Chitin_bind_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGAAAGGCAGTGAGCTGCCAACATAAGC |
| Internal bar code: | CCGGCTTTATGCTGCTTACGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 99.4583 |
| LEAP-Seq n confirming: | 2387 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTATTGCCACATAATGCC |
| Suggested primer 2: | TTCAACAATTGTGCGGATGT |