| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144378 |
| Chromosome: | chromosome 6 |
| Location: | 2860313 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271900 | (1 of 1) 1.1.99.37 - Methanol dehydrogenase (nicotinoprotein) / Nicotinoprotein methanol dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGGGAGGGCGTAGTCAGGTAAAGGAAGG |
| Internal bar code: | GAGCTGGTATTCACGTGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 916 |
| LEAP-Seq percent confirming: | 99.6169 |
| LEAP-Seq n confirming: | 6241 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTTCAGGTGTGGCAGTA |
| Suggested primer 2: | CAGAGCCTGTGTCCTTGTGA |