| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.144398 |
| Chromosome: | chromosome 13 |
| Location: | 2160846 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578100 | NUP205 | (1 of 1) K14310 - nuclear pore complex protein Nup205 (NUP205, NUP192); Nucleoporin 205 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCCCTGCAGCTGCAGCTGCTGCTGGC |
| Internal bar code: | GGGGTGAGTCGAGAGTGTAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 52 |
| LEAP-Seq percent confirming: | 84.375 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGCCAAACTAAACCGTA |
| Suggested primer 2: | GTTGGAGATGGGGAGTGAGA |