Insertion junction: LMJ.RY0402.144401_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.144401
Chromosome:chromosome 16
Location:902243
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g648300 PHT6,PHT4-6,PHT4F Sodium-dependent phosphate transporter, major facilitator superfamily; (1 of 3) PTHR11662:SF243 - ANION TRANSPORTER 6, CHLOROPLASTIC-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCGGTTGTGTTTGTGTGGTTGTGGTTGT
Internal bar code:CGACTGATTAAGAAGAGAGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:802
LEAP-Seq percent confirming:99.85
LEAP-Seq n confirming:2662
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TGATGAACCGTCACCTCGTA
Suggested primer 2:CATCCTCAATCCCACAGCTT