| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.144406 |
| Chromosome: | chromosome 14 |
| Location: | 3189335 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629102 | (1 of 2) PTHR11731//PTHR11731:SF117 - PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED // GLUTAMYL ENDOPEPTIDASE, CHLOROPLASTIC-RELATED | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCTTCCCCGCTCTCGCCCTGTTGCCC |
| Internal bar code: | GCTTAGCTACTTGGGTCATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 84 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCGAAGCAAAAGACTTC |
| Suggested primer 2: | GCCACTGACAAGATCAGCAA |