Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.144410 |
Chromosome: | chromosome_16 |
Location: | 7484102 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g687182 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATCTATCCATCGCAGCCGTACGACCTGAGC |
Internal bar code: | CCTGGGCCCCGAGTAGCCCTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 838 |
LEAP-Seq percent confirming: | 99.5819 |
LEAP-Seq n confirming: | 4287 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCACAGCTTATGATCCGCA |
Suggested primer 2: | GCTTACTTGTGAGGGCTTGC |