| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.144431 |
| Chromosome: | chromosome 12 |
| Location: | 6154491 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGCAGGGCTTTCCACGTCTGGCAAGA |
| Internal bar code: | TGGCATGCCCGTGCGCACATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 498 |
| LEAP-Seq percent confirming: | 93.7888 |
| LEAP-Seq n confirming: | 1057 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGACAATTCCTGCTCCTC |
| Suggested primer 2: | TATGCAGGAGGTGCTGTGAG |