| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.144450 |
| Chromosome: | chromosome 12 |
| Location: | 8674090 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546550 | FEA1 | Fe-assimilating protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAATCACGCCCCTGCCCGCTCCGCTCG |
| Internal bar code: | CAGTCGGGACTCGACACTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 99.751 |
| LEAP-Seq n confirming: | 3605 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTAACACTTCCAAGCAT |
| Suggested primer 2: | CCCATACGTAACACAGGGCT |